Second-Year Biochar Residue and NPK Fertilizer Effects on Soil Mesofauna and Microbial Activity in Patchouli (Pogostemon cablin Benth.)

Any  Kusumastuti; Made Same; Nurhasanah; AH. Maftuh Hafidh Zuhdi; Dian Latifathul  Mar'ah

doi:10.20527/actasolum.v4i2.3560

Authors

Any Kusumastuti State Polytechnic of Lampung, Bandar Lampung, Lampung 35141, Indonesia https://orcid.org/0000-0003-4668-7779
Made Same State Polytechnic of Lampung, Bandar Lampung, Lampung 35141, Indonesia
Nurhasanah State Polytechnic of Lampung, Bandar Lampung, Lampung 35141, Indonesia
AH. Maftuh Hafidh Zuhdi State Polytechnic of Lampung, Bandar Lampung, Lampung 35141, Indonesia https://orcid.org/0009-0008-1451-9141
Dian Latifathul Mar'ah State Polytechnic of Lampung, Bandar Lampung, Lampung 35141, Indonesia

DOI:

https://doi.org/10.20527/actasolum.v4i2.3560

Keywords:

Biochar, Mesofauna, Microbial respiration, NPK application, Patchouli cultivation

Abstract

Second-year biochar residue represents an understudied aspect of long-term soil amendment management, as its persistence in soil may influence biological communities differently over time. This study aimed to analyze the effects of second-year biochar residue and NPK fertilizer rates on soil mesofauna abundance and soil microbial activity in patchouli (Pogostemon cablin Benth.) cultivation. The experiment was arranged in a split-plot design with three replications. The main plot factor was second-year biochar residue rates (0, 10, and 20 ton ha^-1), and the subplot factor was NPK fertilizer rates (0, 350, and 700 kg ha^-1). Second-year biochar residue had no significant effect on soil mesofauna population, diversity index, dominance index, or microbial activity, while NPK fertilizer at 700 kg ha^-1 significantly increased mesofauna population (24.33 individuals dm^-³), diversity index (0.28), and dominance index (0.022). A total of 501 mesofauna individuals were identified, dominated by Acarina (67.3%) and Collembola (28.9%). Correlation analysis revealed strong positive relationships between mesofauna variables and soil physical factors, particularly temperature (r = 0.74) and moisture (r = 0.83), while soil chemical properties showed weak correlations. No significant interactions were observed between biochar residue and NPK fertilizer. These findings indicate that biochar’s stimulatory effects on soil biota diminish substantially after the first year. In contrast, NPK fertilization at 700 kg ha^-1 effectively enhances soil mesofauna communities, which serve as indicators of soil biological health in patchouli cultivation systems.

References

Agegnehu G, Bass, A.M., Nelson, P.N., Bird, M.I. 2016. Benefits of biochar, compost and biochar-compost for soil quality, maize yield and greenhouse gas emissions in a tropical agricultural soil. Science of the Total Environment 543, 295-306. https://doi.org/10.1016/j.scitotenv.2015.11.054

Anas, I. 1989. Biologi Tanah dalam Praktek. Institut Pertanian Bogor, Bogor. (in Indonesian)

Bedano, J.C., Domínguez, A., Arolfo, R., Wall, D.H. 2016. Effect of good agricultural practices under no-till on litter and soil invertebrates in areas with different soil types. Soil and Tillage Research 158, 100-109. https://doi.org/10.1016/j.still.2015.12.005

Blanco-Canqui, H. 2017. Biochar and soil physical properties. Soil Science Society of America Journal 81(4), 687-711. https://doi.org/10.2136/sssaj2017.01.0017

Coulibaly, S.F.M., Winck, B.R., Akpa-Vinceslas, M., Mignot, L., Legras, M., Forey, E., Chauvat, M. 2017. Functional assemblages of Collembola determine soil microbial communities and associated functions. Frontiers in Environmental Science 5, 81. https://doi.org/10.3389/fenvs.2017.00081

Dai, Z., Zhang, X., Tang, C., Muhammad, N., Wu, J., Brookes, P.C., Xu, J. 2021. Potential role of biochars in decreasing soil acidification -A critical review. Science of the Total Environment 753, 142043. https://doi.org/10.1016/j.scitotenv.2020.142043

Gao, M., Taylor, M.K., Callaham Jr, M.A. 2020. Trophic ecology of soil mesofauna in a successional gradient of montane forests. Soil Biology and Biochemistry 148, 107867. https://doi.org/10.1016/j.soilbio.2020.107867

Kementerian Pertanian. 2024. Outlook Komoditas Perkebunan Nilam. Pusat Data dan Sistem Informasi Pertanian, Jakarta. (in Indonesian)

Krebs, C.J. 1989. Ecological Methodology. Harper and Row Publishers, New York.

Kusumastuti, A., Indrawat,i W., Kurniawan, A. 2022. Keanekaragaman mesofauna tanah dan aktivitas mikroorganisme tanah pada vegetasi nilam di berbagai dosis biochar dan pupuk majemuk NPK. Agriprima 6(2), 145-162. https://doi.org/10.25047/agriprima.v6i2.488 (in Indonesian)

Kuzyakov, Y., Subbotina, I., Chen, H., Bogomolova, I., Xu, X. 2009. Black carbon decomposition and incorporation into soil microbial biomass estimated by ¹⁴C labeling. Soil Biology and Biochemistry 41(2), 210-219. https://doi.org/10.1016/j.soilbio.2008.10.016

Lehmann, J., Cowie, A., Masiello, C.A., Kammann, C., Woolf, D., Amonette, J.E., Cayuela, M.L., Camps-Arbestain, M., Whitman, T. 2021. Biochar in climate change mitigation. Nature Geoscience 14(12), 883-892. https://doi.org/10.1038/s41561-021-00852-8

Magurran, A.E. 2004. Measuring Biological Diversity. Blackwell Publishing, Oxford.

Menta, C., Remelli, S. 2020. Soil health and arthropods: From complex system to worthwhile investigation. Insects 11(1), 54. https://doi.org/10.3390/insects11010054

Nisa, K., Sufardi, S., Rusdi, M., Indra, I. 2024. Soil quality index and patchouli yields on various cropping systems in Aceh province, Indonesia: Case study in Aceh Barat Regency. Case Studies in Chemical and Environmental Engineering 10, 100798. https://doi.org/10.1016/j.cscee.2024.100798

Odum, E.P. 1983. Basic Ecology. Saunders College Publishing, Philadelphia.

Palansooriya, K.N., Ok, Y.S., Awad, Y.M., Lee, S.S., Sung, J.K., Koutsospyros, A., Moon, D.H. 2019. Impacts of biochar application on upland agriculture: A review. Journal of Environmental Management 234, 52-64. https://doi.org/10.1016/j.jenvman.2018.12.085

Potapov, A.M., Tiunov, A.V., Scheu, S. 2021. Uncovering trophic positions and food resources of soil animals using bulk natural stable isotope composition. Biological Reviews 94(1), 37-59. https://doi.org/10.1111/brv.12434

Sun, H., Lu, H., Chu, L., Shao, H., Shi, W. 2019. Biochar applied with appropriate rates can reduce N leaching, keep N retention and not increase NH₃ volatilization in a coastal saline soil. Science of the Total Environment 575, 820-825. https://doi.org/10.1016/j.scitotenv.2016.09.137

Trivedi, P., Delgado-Baquerizo, M., Trivedi, C., Hu, H., Anderson, I.C., Jeffries, T.C., Zhou, J., Singh, B.K. 2016. Microbial regulation of the soil carbon cycle: Evidence from gene-enzyme relationships. The ISME Journal 10, 2593-2604. https://doi.org/10.1038/ismej.2016.65

Wang, J., Xiong, Z., Kuzyakov, Y. 2016. Biochar stability in soil: Meta-analysis of decomposition and priming effects. GCB Bioenergy 8(3), 512-523. https://doi.org/10.1111/gcbb.12266

Wang, X., Zeeshan Ul Haq, M., Yu, J., Liu, Y., Yang, H., Cui, H., Yang, D., Wu, Y. 2024. Continuous cropping of patchouli alleviate soil properties, enzyme activities, and bacterial community structures. Plants 13(24), 3481. https://doi.org/10.3390/plants13243481

Xiao, L., Yuan, G., Feng, L., Bi, D., Wei, J. 2018. Soil properties and the growth of wheat (Triticum aestivum L.) and maize (Zea mays L.) in response to reed (Phragmites communis) biochar use in a salt-affected soil in the Yellow River Delta. Agriculture, Ecosystems & Environment 258, 51-60. https://doi.org/10.1016/j.agee.2018.02.010

Zhu, Z., Bai, Y., Lv, M., Tian, G., Zhang, X., Li, L., Jiang, Y., Ge, S. 2020. Soil fertility, microbial biomass, and microbial functional diversity responses to four years fertilization in an apple orchard in North China. Horticultural Plant Journal 6(4), 223-230. https://doi.org/10.1016/j.hpj.2020.06.002